GATC-specific restriction and modification systems in treponemes
نویسندگان
چکیده
منابع مشابه
Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF.
The sequence specificities of three Bacillus subtilis restriction/modification systems were established: (i) BsuM (CTCGAG), an isoschizomer to XhoI; (ii) BsuE (CGCG), an isoschizomer to FnuDII; and (iii) BsuF (CCGG), an isoschizomer to MspI, HpaII. The BsuM modification enzyme methylates the 3' cytosine of the recognition sequence. The BsuF modification enzyme methylates the 5' cytosine of the ...
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Two plasmid-carried restriction-modification (R-M) systems, EcoRI (from pMB1 of Escherichia coli) and Bsp6I (from pXH13 of Bacillus sp. strain RFL6), enhance plasmid segregational stability in E. coli and Bacillus subtilis, respectively. Inactivation of the endonuclease or the presence of the methylase in trans abolish the stabilizing activity of the R-M systems. We propose that R-M systems med...
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متن کاملMethylation sensitivity of restriction enzymes interacting with GATC sites.
924 BioTechniques Vol. 34, No. 5 (2003) inates the residual ribonuclease activity, although the effect of this treatment on the subsequent experiments should be checked. An inhibitor of RNase, such as Rnasin, can also be added to further enzymatic reactions. This study has described a degrading activity in RNA preparations from ovine, bovine, and porcine brain and ovine liver. Further experimen...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2004
ISSN: 0266-8254,1365-2673
DOI: 10.1111/j.1472-765x.2004.01483.x